AMY2 ELISA Kit

Catalog No. ABIN2042835

Product Details AMY2 ELISA Kit

Target: AMY2 (Amylase alpha 2, Pancreatic (AMY2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.37-600 pg/mL

Minimum Detection Limit: 9.37 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 4.1 pg/mL

Application Details

Plate: Pre-coated

Protocol: Amylase Alpha 2, Pancreatic (AMY2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase Alpha 2, Pancreatic (AMY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase Alpha 2, Pancreatic (AMY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase Alpha 2, Pancreatic (AMY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amylase Alpha 2, Pancreatic (AMY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for AMY2

Target: AMY2 (Amylase alpha 2, Pancreatic (AMY2))

Abstract: AMY2 Products