APEX1 ELISA Kit

Catalog No. ABIN2042915

Product Details APEX1 ELISA Kit

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: APEX Nuclease 1 (APEX1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to APEX Nuclease 1 (APEX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APEX Nuclease 1 (APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APEX Nuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of APEX Nuclease 1 (APEX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX Nuclease 1 (APEX1) (APEX1 Products)

Synonyms: apex1 ELISA Kit, MGC76280 ELISA Kit, MGC79645 ELISA Kit, MGC78928 ELISA Kit, APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Apex ELISA Kit, REF-1 ELISA Kit, BAP1 ELISA Kit, apex1a ELISA Kit, hm:zehn1532 ELISA Kit, wu:fc13d11 ELISA Kit, zgc:66204 ELISA Kit, Ref-1 ELISA Kit, apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog ELISA Kit, DNA-(apurinic or apyrimidinic site) lyase ELISA Kit, apurinic/apyrimidinic endonuclease 1 ELISA Kit, APEX1 ELISA Kit, apex1 ELISA Kit, apex1.L ELISA Kit, CNG03640 ELISA Kit, CNH01280 ELISA Kit, nth ELISA Kit, Spirs_4119 ELISA Kit, Palpr_0103 ELISA Kit, Apex1 ELISA Kit

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus