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ARSF ELISA Kit

ARSF Reactivity: Human Colorimetric Sandwich ELISA 0.31-20 ng/mL
Catalog No. ABIN2042947
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    ARSF (Arylsulfatase F (ARSF))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.31-20 ng/mL
    Minimum Detection Limit
    0.31 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.112 ng/mL
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  • Plate
    Pre-coated
    Protocol
    Arylsulfatase F (ARSF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase F (ARSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase F (ARSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase F (ARSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arylsulfatase F (ARSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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    ARSF (Arylsulfatase F (ARSF))
    Abstract
    ARSF Products
    Synonyms
    ASF ELISA Kit, ARSF ELISA Kit, arylsulfatase F ELISA Kit, ARSF ELISA Kit
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