ATP1B4 ELISA Kit

Catalog No. ABIN2042977

Product Details ATP1B4 ELISA Kit

Target: ATP1B4 (ATPase, Na+/K+ Transporting, beta 4 Polypeptide (ATP1B4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ATP1B4

Target: ATP1B4 (ATPase, Na+/K+ Transporting, beta 4 Polypeptide (ATP1B4))

Alternative Name: ATPase, Na+/K+ Transporting beta 4 Polypeptide (ATP1b4) (ATP1B4 Products)

Synonyms: 1110020B02Rik ELISA Kit, Ac2-628 ELISA Kit, ATPase, (Na+)/K+ transporting, beta 4 polypeptide ELISA Kit, ATPase Na+/K+ transporting family member beta 4 ELISA Kit, ATPase, Na+/K+ transporting, beta 4 polypeptide L homeolog ELISA Kit, Atp1b4 ELISA Kit, ATP1B4 ELISA Kit, atp1b4.L ELISA Kit

Pathways: Thyroid Hormone Synthesis, Proton Transport