HLAG ELISA Kit
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- Target See all HLAG ELISA Kits
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.31-20 ng/mL
- Minimum Detection Limit
- 0.31 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.136 ng/mL
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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA Kit
HLAG Reactivity: Human Colorimetric Sandwich ELISA 0.38 ng/mL - 24 ng/mL Plasma, Serum, Tissue Homogenate
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) ELISA KitHLAG Reactivity: Human Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
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- Plate
- Pre-coated
- Protocol
- Beta-2-Microglobulin (b2M) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all HLAG ELISA Kits
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- Alternative Name
- b2-Microglobulin (b2M) (HLAG Products)
- Synonyms
- MHC-G ELISA Kit, B-F ELISA Kit, B-F-S04 ELISA Kit, B-F-S05 ELISA Kit, B-F-S06 ELISA Kit, B-F-S07 ELISA Kit, B-FI ELISA Kit, B-FIV ELISA Kit, BF2 ELISA Kit, BFa2 ELISA Kit, BFw-03 ELISA Kit, BFw-05 ELISA Kit, BFz-01 ELISA Kit, major histocompatibility complex, class I, G ELISA Kit, MHC BF1 class I ELISA Kit, HLA-G ELISA Kit, BF1 ELISA Kit
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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