BTK ELISA Kit

Catalog No. ABIN2043023

Product Details BTK ELISA Kit

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Bruton'S Tyrosine Kinase (Btk) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bruton'S Tyrosine Kinase (Btk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bruton'S Tyrosine Kinase (Btk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bruton'S Tyrosine Kinase (Btk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bruton'S Tyrosine Kinase (Btk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: Bruton'S Tyrosine Kinase (Btk) (BTK Products)

Synonyms: BTK ELISA Kit, atk ELISA Kit, bpk ELISA Kit, xla ELISA Kit, imd1 ELISA Kit, agmx1 ELISA Kit, psctk1 ELISA Kit, AGMX1 ELISA Kit, AT ELISA Kit, ATK ELISA Kit, BPK ELISA Kit, IMD1 ELISA Kit, PSCTK1 ELISA Kit, XLA ELISA Kit, AI528679 ELISA Kit, xid ELISA Kit, Bruton tyrosine kinase ELISA Kit, Bruton agammaglobulinemia tyrosine kinase ELISA Kit, BTK ELISA Kit, btk ELISA Kit, Btk ELISA Kit

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling