Cilp2 ELISA Kit

Catalog No. ABIN2043073

Product Details Cilp2 ELISA Kit

Target: Cilp2 (Cartilage Intermediate Layer Protein 2 (Cilp2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.134 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cartilage Intermediate Layer Protein 2 (CILP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cartilage Intermediate Layer Protein 2 (CILP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cartilage Intermediate Layer Protein 2 (CILP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cartilage Intermediate Layer Protein 2 (CILP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cartilage Intermediate Layer Protein 2 (CILP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cilp2

Target: Cilp2 (Cartilage Intermediate Layer Protein 2 (Cilp2))

Abstract: Cilp2 Products

Synonyms: CLIP-2 ELISA Kit, AA407300 ELISA Kit, 1110031K21Rik ELISA Kit, cartilage intermediate layer protein 2 ELISA Kit, CILP2 ELISA Kit, Cilp2 ELISA Kit, cilp2 ELISA Kit