Cathepsin B ELISA Kit

Catalog No. ABIN2043091

Product Details Cathepsin B ELISA Kit

Target: Cathepsin B (CTSB)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cathepsin B (CTSB) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin B (CTSB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin B (CTSB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin B (CTSB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathepsin B (CTSB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Abstract: CTSB Products

Synonyms: APPS ELISA Kit, CPSB ELISA Kit, CB ELISA Kit, apps ELISA Kit, cpsb ELISA Kit, LOC692390 ELISA Kit, CTSB ELISA Kit, cathepsin B ELISA Kit, cathepsin B S homeolog ELISA Kit, CTSB ELISA Kit, Ctsb ELISA Kit, ctsb.S ELISA Kit, LOC692390 ELISA Kit

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades