CD163 ELISA Kit

Catalog No. ABIN2043171

Product Details CD163 ELISA Kit

Target: CD163

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cluster Of Differentiation (CD163) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation (CD163). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation (CD163). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation (CD163), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cluster Of Differentiation (CD163) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD163

Target: CD163

Alternative Name: Cluster Of Differentiation (CD163) (CD163 Products)

Synonyms: DMBT1 ELISA Kit, CD163 ELISA Kit, CD163v2 ELISA Kit, CD163v3 ELISA Kit, M130 ELISA Kit, MM130 ELISA Kit, deleted in malignant brain tumors 1 protein-like ELISA Kit, CD163 molecule ELISA Kit, CD163 antigen ELISA Kit, LOC426820 ELISA Kit, CD163 ELISA Kit, Cd163 ELISA Kit