CRHR1 ELISA Kit

Catalog No. ABIN2043227

Product Details CRHR1 ELISA Kit

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.060 ng/mL

Application Details

Plate: Pre-coated

Protocol: Corticotropin Releasing Hormone Receptor 1 (CRHR1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Receptor 1 (CRHR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Receptor 1 (CRHR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Corticotropin Releasing Hormone Receptor 1 (CRHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Abstract: CRHR1 Products

Synonyms: CRF-R ELISA Kit, CRF-R-1 ELISA Kit, CRF-R1 ELISA Kit, CRF1 ELISA Kit, CRFR-1 ELISA Kit, CRFR1 ELISA Kit, CRH-R-1 ELISA Kit, CRH-R1 ELISA Kit, CRH-R1h ELISA Kit, CRHR ELISA Kit, CRHR1L ELISA Kit, CRHR1f ELISA Kit, Crhr ELISA Kit, crhr1 ELISA Kit, crhr1-A ELISA Kit, CRHR1 ELISA Kit, CRHR1-IT1-CRHR1 ELISA Kit, MGC57346-CRHR1 ELISA Kit, corticotropin releasing hormone receptor 1 ELISA Kit, corticotropin releasing hormone receptor 1, gene 2 S homeolog ELISA Kit, corticotropin-releasing factor receptor 1 ELISA Kit, LINC02210-CRHR1 readthrough ELISA Kit, CRHR1 ELISA Kit, Crhr1 ELISA Kit, crhr1.2.S ELISA Kit, LOC610380 ELISA Kit, LOC100065598 ELISA Kit, LINC02210-CRHR1 ELISA Kit, crhr1 ELISA Kit

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity