CYP1A1 ELISA Kit

Catalog No. ABIN2043269

Product Details CYP1A1 ELISA Kit

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reactivity: Monkey

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.056 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cytochrome P450 1A1 (CYP1A1) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A1 (CYP1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A1 (CYP1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A1 (CYP1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytochrome P450 1A1 (CYP1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CYP1A1

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Alternative Name: Cytochrome P450 1A1 (CYP1A1) (CYP1A1 Products)

Synonyms: AHH ELISA Kit, AHRR ELISA Kit, CP11 ELISA Kit, CYP1 ELISA Kit, P1-450 ELISA Kit, P450-C ELISA Kit, P450DX ELISA Kit, Cyp1a2 ELISA Kit, P450-1 ELISA Kit, cyp1a1 ELISA Kit, CYP1A1 ELISA Kit, CYPIA1 ELISA Kit, Cyp45c ELISA Kit, Cypc45c ELISA Kit, P-450MC ELISA Kit, wu:fb63b04 ELISA Kit, zfCYP1A ELISA Kit, zgc:109747 ELISA Kit, ahh ELISA Kit, ahrr ELISA Kit, cp11 ELISA Kit, cyp1 ELISA Kit, cyp1a ELISA Kit, p1-450 ELISA Kit, p450-c ELISA Kit, p450dx ELISA Kit, cytochrome P450 family 1 subfamily A member 1 ELISA Kit, cytochrome P450 1A1 ELISA Kit, cytochrome P450, family 1, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 1, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450 family 1 subfamily D polypeptide 1 ELISA Kit, cytochrome P4501A1 ELISA Kit, polycyclic hydrocarbon-inducible cytochrome P450c ELISA Kit, cytochrome P450, family 1, subfamily A ELISA Kit, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 ELISA Kit, cytochrome P450 family 1 subfamily A member 1 L homeolog ELISA Kit, CYP1A1 ELISA Kit, CpipJ_CPIJ010542 ELISA Kit, Cyp1a1 ELISA Kit, cyp1d1 ELISA Kit, LOC100328613 ELISA Kit, cyp1a ELISA Kit, LOC102129994 ELISA Kit, cyp1a1.L ELISA Kit

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha