CYP27A1 ELISA Kit

Catalog No. ABIN2043271

Product Details CYP27A1 ELISA Kit

Target: CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cytochrome P450 27A1 (CYP27A1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CYP27A1

Target: CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))

Alternative Name: Cytochrome P450 27A1 (CYP27A1) (CYP27A1 Products)

Synonyms: CP27 ELISA Kit, CTX ELISA Kit, CYP27 ELISA Kit, Cyp27 ELISA Kit, P450C27 ELISA Kit, 1300013A03Rik ELISA Kit, cytochrome P450 family 27 subfamily A member 1 ELISA Kit, cytochrome P450, family 27, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily A, polypeptide 1 ELISA Kit, sterol 26-hydroxylase, mitochondrial ELISA Kit, CYP27A1 ELISA Kit, Cyp27a1 ELISA Kit, cyp27a1 ELISA Kit