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DNMT3B ELISA Kit

DNMT3B Reactivity: Human Colorimetric Sandwich ELISA 0.31-20 ng/mL
Catalog No. ABIN2043359
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    DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))
    Reactivity
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.31-20 ng/mL
    Minimum Detection Limit
    0.31 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.125 ng/mL
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  • Plate
    Pre-coated
    Protocol
    DNA Methyltransferase 3B (DNMT3B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 3B (DNMT3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 3B (DNMT3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 3B (DNMT3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DNA Methyltransferase 3B (DNMT3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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    DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))
    Alternative Name
    DNA Methyltransferase 3B (DNMT3B) (DNMT3B Products)
    Synonyms
    DNMT3B ELISA Kit, LOC100218113 ELISA Kit, ICF ELISA Kit, ICF1 ELISA Kit, M.HsaIIIB ELISA Kit, MmuIIIB ELISA Kit, cb91 ELISA Kit, dnmt3bl ELISA Kit, dnmt7 ELISA Kit, sb:cb91 ELISA Kit, wu:fb16h07 ELISA Kit, DNA methyltransferase 3 beta ELISA Kit, DNA methyltransferase 3B ELISA Kit, DNA (cytosine-5-)-methyltransferase 3 beta, duplicate a ELISA Kit, DNMT3B ELISA Kit, Dnmt3b ELISA Kit, dnmt3b ELISA Kit, dnmt3ba ELISA Kit
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