Epigen ELISA Kit

Catalog No. ABIN2043407

Product Details Epigen ELISA Kit

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Epigen (EPG) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epigen (EPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epigen (EPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epigen (EPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Epigen (EPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Epigen

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Alternative Name: Epigen (EPG) (EPGN Products)

Synonyms: ALGV3072 ELISA Kit, EPG ELISA Kit, PRO9904 ELISA Kit, 2310069M11Rik ELISA Kit, epigen ELISA Kit, RGD1560084 ELISA Kit, epithelial mitogen ELISA Kit, EPGN ELISA Kit, Epgn ELISA Kit

Pathways: RTK Signaling, EGFR Signaling Pathway