FGF15 ELISA Kit (Fibroblast Growth Factor 15)

Details for Product FGF15 ELISA Kit No. ABIN2043463, Supplier: Log in to see
Antigen
  • FGF19
  • Fgf15
  • fibroblast growth factor 15
  • fibroblast growth factor 19
  • Fgf15
  • Fgf19
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
12
5
Method Type
Sandwich ELISA
Detection Range
15.62-1000 pg/mL
Minimum Detection Limit
15.62 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 5.7 pg/mL
Plate Pre-coated
Protocol Fibroblast Growth Factor 15 (FGF15) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 15 (FGF15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 15 (FGF15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 15 (FGF15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 15 (FGF15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C