FGF2 ELISA Kit

Catalog No. ABIN2043469

Product Details FGF2 ELISA Kit

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.35-1000 pg/mL

Minimum Detection Limit: 12.35 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 4.27 pg/mL

Application Details

Plate: Pre-coated

Protocol: Fibroblast Growth Factor 2, Basic (FGF2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 2, Basic (FGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fibroblast Growth Factor 2, Basic (FGF2) (FGF2 Products)

Synonyms: BFGF ELISA Kit, FGF-2 ELISA Kit, FGFB ELISA Kit, HBGF-2 ELISA Kit, Fgf-2 ELISA Kit, Fgfb ELISA Kit, bFGF ELISA Kit, fibroblast growth factor 2 ELISA Kit, fibroblast growth factor 2 (basic) ELISA Kit, FGF2 ELISA Kit, Fgf2 ELISA Kit, fgf2 ELISA Kit

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins