FAH ELISA Kit

Catalog No. ABIN2043517

Product Details FAH ELISA Kit

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Fumarylacetoacetate Hydrolase (FAH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FAH

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Alternative Name: Fumarylacetoacetate Hydrolase (FAH) (FAH Products)

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