CD235a/GYPA ELISA Kit

Catalog No. ABIN2043581

Product Details CD235a/GYPA ELISA Kit

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.134 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glycophorin A (GYPA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycophorin A (GYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycophorin A (GYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycophorin A (GYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycophorin A (GYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Abstract: GYPA Products

Synonyms: GYPA ELISA Kit, gpa ELISA Kit, AI853584 ELISA Kit, CD235a ELISA Kit, GPA ELISA Kit, GPErik ELISA Kit, GPSAT ELISA Kit, HGpMiV ELISA Kit, HGpMiXI ELISA Kit, HGpSta(C) ELISA Kit, MN ELISA Kit, MNS ELISA Kit, PAS-2 ELISA Kit, glycophorin A (MNS blood group) ELISA Kit, glycophorin A ELISA Kit, GYPA ELISA Kit, Gypa ELISA Kit

Pathways: Maintenance of Protein Location