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HTRA4 ELISA Kit

HTRA4 Reactivity: Human Colorimetric Sandwich ELISA 0.78-50 ng/mL
Catalog No. ABIN2043719
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    HTRA4 (HtrA Serine Peptidase 4 (HTRA4))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78-50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.28 ng/mL
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  • Plate
    Pre-coated
    Protocol
    HtrA Serine Peptidase 4 (HTRA4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HtrA Serine Peptidase 4 (HTRA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HtrA Serine Peptidase 4 (HTRA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HtrA Serine Peptidase 4 (HTRA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of HtrA Serine Peptidase 4 (HTRA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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    HTRA4 (HtrA Serine Peptidase 4 (HTRA4))
    Abstract
    HTRA4 Products
    Synonyms
    B430206E18Rik ELISA Kit, RGD1306242 ELISA Kit, HtrA serine peptidase 4 ELISA Kit, HTRA4 ELISA Kit, Htra4 ELISA Kit
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