HAase ELISA Kit

Catalog No. ABIN2043721

Product Details HAase ELISA Kit

Target: HAase (Hyaluronidase (HAase))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 36 pg/mL

Application Details

Plate: Pre-coated

Protocol: Hyaluronidase (HAase) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase (HAase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronidase (HAase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronidase (HAase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hyaluronidase (HAase) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HAase

Target: HAase (Hyaluronidase (HAase))

Abstract: HAase Products

Synonyms: hyaluronidase ELISA Kit, Hyaluronidase ELISA Kit, hylP ELISA Kit, chhy-1 ELISA Kit, hylP.1 ELISA Kit, hylP.4 ELISA Kit

Pathways: Glycosaminoglycan Metabolic Process