HYAL1 ELISA Kit

Catalog No. ABIN2043723

Product Details HYAL1 ELISA Kit

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.55 ng/mL

Application Details

Plate: Pre-coated

Protocol: Hyaluronoglucosaminidase 1 (HYAL1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronoglucosaminidase 1 (HYAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hyaluronoglucosaminidase 1 (HYAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: Hyaluronoglucosaminidase 1 (HYAL1) (HYAL1 Products)

Synonyms: HYAL-1 ELISA Kit, LUCA1 ELISA Kit, NAT6 ELISA Kit, Hya1 ELISA Kit, Hyal-1 ELISA Kit, hyaluronoglucosaminidase 1 ELISA Kit, HYAL1 ELISA Kit, Hyal1 ELISA Kit

Pathways: Glycosaminoglycan Metabolic Process