IRS3 ELISA Kit
IRS3
Reactivity: Rat
Colorimetric
Sandwich ELISA
0.62-40 ng/mL
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- IRS3 (Insulin Receptor Substrate 3 (IRS3))
- Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.62-40 ng/mL
- Minimum Detection Limit
- 0.62 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.227 ng/mL
- Featured
- Discover our best selling IRS3 ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Insulin Receptor Substrate 3 (IRS3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 3 (IRS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 3 (IRS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 3 (IRS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Receptor Substrate 3 (IRS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all IRS3 products
- IRS3 (Insulin Receptor Substrate 3 (IRS3))
- Abstract
- IRS3 Products
- Synonyms
- AA408304 ELISA Kit, insulin receptor substrate 3 ELISA Kit, Irs3 ELISA Kit
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