IRF5 ELISA Kit

Catalog No. ABIN2043785

Product Details IRF5 ELISA Kit

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.048 ng/mL

Application Details

Plate: Pre-coated

Protocol: Interferon Regulatory Factor 5 (IRF5) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 5 (IRF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 5 (IRF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 5 (IRF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interferon Regulatory Factor 5 (IRF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Abstract: IRF5 Products

Synonyms: IRF5 ELISA Kit, irf5 ELISA Kit, SLEB10 ELISA Kit, AW491843 ELISA Kit, mirf5 ELISA Kit, im:7155364 ELISA Kit, zgc:76986 ELISA Kit, interferon regulatory factor 5 ELISA Kit, interferon regulatory factor 5 L homeolog ELISA Kit, IRF5 ELISA Kit, irf5 ELISA Kit, Irf5 ELISA Kit, irf5.L ELISA Kit

Pathways: TLR Signaling, Autophagy