JAM3 ELISA Kit

Catalog No. ABIN2043833

Product Details JAM3 ELISA Kit

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 6.5 pg/mL

Application Details

Plate: Pre-coated

Protocol: Junctional Adhesion Molecule 3 (JAM3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junctional Adhesion Molecule 3 (JAM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junctional Adhesion Molecule 3 (JAM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junctional Adhesion Molecule 3 (JAM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Junctional Adhesion Molecule 3 (JAM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for JAM3

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Abstract: JAM3 Products

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