Lipase A ELISA Kit (Lipase A, Lysosomal Acid, Cholesterol Esterase)

Details for Product LIPA ELISA Kit No. ABIN2043893, Supplier: Log in to see
Antigen
  • cesd
  • lal
  • LIPA
  • CESD
  • LAL
  • Chole
  • Chole2
  • Lip1
  • AA960673
  • Lal
  • Lip-1
  • lipase A, lysosomal acid type
  • lipase A, lysosomal acid, cholesterol esterase
  • lipase A, lysosomal acid, cholesterol esterase L homeolog
  • lysosomal acid lipase A
  • LIPA
  • lipa
  • lipa.L
  • Lipa
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
7
1
1
Method Type
Sandwich ELISA
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.117 ng/mL
Alternative Name Lipase A, Lysosomal Acid (LIPA) (LIPA ELISA Kit Abstract)
Plate Pre-coated
Protocol Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C