HLA-DRB1 ELISA Kit

Catalog No. ABIN2043919

Product Details HLA-DRB1 ELISA Kit

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.72 ng/mL

Application Details

Plate: Pre-coated

Protocol: Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HLA-DRB1

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Alternative Name: Major Histocompatibility Complex Class II DR beta 1 (MHCDRb1) (HLA-DRB1 Products)

Synonyms: MGC108235 ELISA Kit, DRB1 ELISA Kit, DRw10 ELISA Kit, HLA-DR1B ELISA Kit, HLA-DRB ELISA Kit, SS1 ELISA Kit, major histocompatibility complex, class II, DR beta 1 ELISA Kit, hla-drb1 ELISA Kit, HLA-DRB1 ELISA Kit

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints