MT2 ELISA Kit

Catalog No. ABIN2043957

Product Details MT2 ELISA Kit

Target: MT2 (Metallothionein 2 (MT2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: Metallothionein 2 (MT2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metallothionein 2 (MT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metallothionein 2 (MT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metallothionein 2 (MT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Metallothionein 2 (MT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MT2

Target: MT2 (Metallothionein 2 (MT2))

Abstract: MT2 Products

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Pathways: Transition Metal Ion Homeostasis