MT3 ELISA Kit

Catalog No. ABIN2043959

Product Details MT3 ELISA Kit

Target: MT3 (Metallothionein 3 (MT3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.074 ng/mL

Application Details

Plate: Pre-coated

Protocol: Metallothionein 3 (MT3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metallothionein 3 (MT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metallothionein 3 (MT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metallothionein 3 (MT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Metallothionein 3 (MT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MT3

Target: MT3 (Metallothionein 3 (MT3))

Abstract: MT3 Products

Synonyms: MT1 ELISA Kit, GIF ELISA Kit, GIFB ELISA Kit, GRIF ELISA Kit, ZnMT3 ELISA Kit, Mt-3 ELISA Kit, MT-III ELISA Kit, ATMT3 ELISA Kit, metallothionein 3 ELISA Kit, MT3 ELISA Kit, MT-3 ELISA Kit, metallothionein 3 ELISA Kit, MT3 ELISA Kit, Mt3 ELISA Kit

Pathways: Hormone Transport, Transition Metal Ion Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus