MARCKS ELISA Kit

Catalog No. ABIN2044021

Product Details MARCKS ELISA Kit

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.55 ng/mL

Application Details

Plate: Pre-coated

Protocol: Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) (MARCKS Products)

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