SGSH ELISA Kit

Catalog No. ABIN2044033

Product Details SGSH ELISA Kit

Target: SGSH (N-Sulfoglucosamine Sulfohydrolase (SGSH))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.056 ng/mL

Application Details

Plate: Pre-coated

Protocol: N-Sulfoglucosamine Sulfohydrolase (SGSH) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Sulfoglucosamine Sulfohydrolase (SGSH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Sulfoglucosamine Sulfohydrolase (SGSH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Sulfoglucosamine Sulfohydrolase (SGSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-Sulfoglucosamine Sulfohydrolase (SGSH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SGSH

Target: SGSH (N-Sulfoglucosamine Sulfohydrolase (SGSH))

Abstract: SGSH Products

Synonyms: HSS ELISA Kit, MPS3A ELISA Kit, SFMD ELISA Kit, 4632406A19Rik ELISA Kit, N-sulfoglucosamine sulfohydrolase ELISA Kit, N-sulfoglucosamine sulfohydrolase (sulfamidase) ELISA Kit, SGSH ELISA Kit, Sgsh ELISA Kit

Pathways: Glycosaminoglycan Metabolic Process