NCF1 ELISA Kit

Catalog No. ABIN2044091

Product Details NCF1 ELISA Kit

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Neutrophil Cytosolic Factor 1 (NCF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: Neutrophil Cytosolic Factor 1 (NCF1) (NCF1 Products)

Synonyms: NCF1A ELISA Kit, NOXO2 ELISA Kit, SH3PXD1A ELISA Kit, p47phox ELISA Kit, Ncf-1 ELISA Kit, p47 ELISA Kit, neutrophil cytosolic factor 1 ELISA Kit, NCF1 ELISA Kit, Ncf1 ELISA Kit

Pathways: PI3K-Akt Signaling