NONO ELISA Kit

Catalog No. ABIN2044103

Product Details NONO ELISA Kit

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: Non POU Domain Containing Octamer Binding Protein (NONO) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: Non POU Domain Containing Octamer Binding Protein (NONO) (NONO Products)

Synonyms: NMT55 ELISA Kit, NRB54 ELISA Kit, P54 ELISA Kit, P54NRB ELISA Kit, nmt55 ELISA Kit, nrb54 ELISA Kit, p54nrb ELISA Kit, xp54nrb ELISA Kit, AA407051 ELISA Kit, AV149256 ELISA Kit, nonA ELISA Kit, non-POU domain containing octamer binding ELISA Kit, non-POU domain containing, octamer-binding ELISA Kit, non-POU domain containing, octamer binding L homeolog ELISA Kit, non-POU-domain-containing, octamer binding protein ELISA Kit, NONO ELISA Kit, Nono ELISA Kit, nono.L ELISA Kit