MGMT ELISA Kit
-
- Target See all MGMT ELISA Kits
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
-
Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 78.12-5000 pg/mL
- Minimum Detection Limit
- 78.12 pg/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 27 pg/mL
- Featured
- Discover our best selling MGMT ELISA Kit
- Top Product
- Discover our top product MGMT ELISA Kit
-
-
- Plate
- Pre-coated
- Protocol
- O-6-Methylguanine DNA Methyltransferase (MGMT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C/-20 °C
-
- Target See all MGMT ELISA Kits
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- Alternative Name
- O-6-Methylguanine DNA Methyltransferase (MGMT) (MGMT Products)
- Synonyms
- AGT ELISA Kit, AI267024 ELISA Kit, Agat ELISA Kit, O-6-methylguanine-DNA methyltransferase ELISA Kit, MGMT ELISA Kit, Mgmt ELISA Kit
- Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
-