PLA2G5 ELISA Kit

Catalog No. ABIN2044221

Product Details PLA2G5 ELISA Kit

Target: PLA2G5 (phospholipase A2, Group V (PLA2G5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 37 pg/mL

Application Details

Plate: Pre-coated

Protocol: Phospholipase A2, Group V (PLA2G5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group V (PLA2G5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group V (PLA2G5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group V (PLA2G5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group V (PLA2G5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PLA2G5

Target: PLA2G5 (phospholipase A2, Group V (PLA2G5))

Abstract: PLA2G5 Products

Synonyms: PLA2 ELISA Kit, sPLA2 ELISA Kit, FRFB ELISA Kit, GV-PLA2 ELISA Kit, PLA2-10 ELISA Kit, hVPLA(2) ELISA Kit, phospholipase A2 group V ELISA Kit, phospholipase A2, group V ELISA Kit, PLA2G5 ELISA Kit, Pla2g5 ELISA Kit

Pathways: Inositol Metabolic Process