PRM1 ELISA Kit

Catalog No. ABIN2044307

Product Details PRM1 ELISA Kit

Target: PRM1 (Protamine 1 (PRM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protamine 1 (PRM1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protamine 1 (PRM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protamine 1 (PRM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protamine 1 (PRM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protamine 1 (PRM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PRM1

Target: PRM1 (Protamine 1 (PRM1))

Abstract: PRM1 Products

Synonyms: CT94.1 ELISA Kit, P1 ELISA Kit, Prm-1 ELISA Kit, PRM-1 ELISA Kit, protamine ELISA Kit, protamine 1 ELISA Kit, uncharacterized LOC100860923 ELISA Kit, PRM1 ELISA Kit, Prm1 ELISA Kit, LOC100860923 ELISA Kit