P2RY1 ELISA Kit

Catalog No. ABIN2044363

Product Details P2RY1 ELISA Kit

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for P2RY1

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Alternative Name: Purinergic Receptor P2Y, G-Protein Coupled 1 (P2RY1) (P2RY1 Products)

Synonyms: P2Y1 ELISA Kit, P2y ELISA Kit, P2y1 ELISA Kit, purinergic receptor P2Y, G-protein coupled 1 ELISA Kit, purinergic receptor P2Y1 ELISA Kit, P2ry1 ELISA Kit, P2RY1 ELISA Kit

Pathways: Regulation of Carbohydrate Metabolic Process, Feeding Behaviour