PDK1 ELISA Kit

Catalog No. ABIN2044369

Product Details PDK1 ELISA Kit

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.58 ng/mL

Application Details

Plate: Pre-coated

Protocol: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) (PDK1 Products)

Synonyms: ATP5A ELISA Kit, B830012B01 ELISA Kit, D530020C15Rik ELISA Kit, si:zfos-979f1.3 ELISA Kit, 3-phosphoinositide dependent protein kinase-1 ELISA Kit, pyruvate dehydrogenase kinase 1 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) ELISA Kit, pyruvate dehydrogenase kinase, isoenzyme 1 ELISA Kit, pyruvate dehydrogenase kinase, isozyme 1 ELISA Kit, pyruvate dehydrogenase kinase 1 L homeolog ELISA Kit, Pdpk1 ELISA Kit, PDK1 ELISA Kit, ATP5G2 ELISA Kit, Pdk1 ELISA Kit, pdk1 ELISA Kit, pdk1.L ELISA Kit

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect