RNASE7 ELISA Kit

Catalog No. ABIN2044451

Product Details RNASE7 ELISA Kit

Target: RNASE7 (Ribonuclease, RNase A Family, 7 (RNASE7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.61 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease A7 (RNASE7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A7 (RNASE7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A7 (RNASE7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A7 (RNASE7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A7 (RNASE7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASE7

Target: RNASE7 (Ribonuclease, RNase A Family, 7 (RNASE7))

Alternative Name: Ribonuclease A7 (RNASE7) (RNASE7 Products)

Synonyms: ribonuclease A family member 7 ELISA Kit, RNASE7 ELISA Kit