DROSHA ELISA Kit

Catalog No. ABIN2044457

Product Details DROSHA ELISA Kit

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease III, Nuclear (RNASEN) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease III, Nuclear (RNASEN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease III, Nuclear (RNASEN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease III, Nuclear (RNASEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease III, Nuclear (RNASEN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DROSHA

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Alternative Name: Ribonuclease III, Nuclear (RNASEN) (DROSHA Products)

Synonyms: im:7150667 ELISA Kit, zgc:158612 ELISA Kit, ETOHI2 ELISA Kit, HSA242976 ELISA Kit, RANSE3L ELISA Kit, RN3 ELISA Kit, RNASE3L ELISA Kit, RNASEN ELISA Kit, 1110013A17Rik ELISA Kit, AI874853 ELISA Kit, Etohi2 ELISA Kit, Rn3 ELISA Kit, Rnasen ELISA Kit, RGD1307626 ELISA Kit, ribonuclease type III, nuclear ELISA Kit, Ribonuclease 3 ELISA Kit, ribonuclease 3 ELISA Kit, drosha ribonuclease III ELISA Kit, drosha, ribonuclease type III ELISA Kit, rnasen ELISA Kit, Thicy_0885 ELISA Kit, Sinme_0866 ELISA Kit, Isova_2087 ELISA Kit, Sphch_0770 ELISA Kit, DROSHA ELISA Kit, Drosha ELISA Kit

Pathways: Regulatory RNA Pathways