RNASEL ELISA Kit

Catalog No. ABIN2044459

Product Details RNASEL ELISA Kit

Target: RNASEL (Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-Dependent) (RNASEL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 7.3 pg/mL

Application Details

Plate: Pre-coated

Protocol: Ribonuclease L (RNASEL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease L (RNASEL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease L (RNASEL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease L (RNASEL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease L (RNASEL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RNASEL

Target: RNASEL (Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-Dependent) (RNASEL))

Alternative Name: Ribonuclease L (RNASEL) (RNASEL Products)

Synonyms: E230029I04Rik ELISA Kit, PRCA1 ELISA Kit, RNS4 ELISA Kit, ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) ELISA Kit, ribonuclease L ELISA Kit, Rnasel ELISA Kit, RNASEL ELISA Kit

Pathways: Hepatitis C