SEMA3A ELISA Kit

Catalog No. ABIN2044505

Product Details SEMA3A ELISA Kit

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.056 ng/mL

Application Details

Plate: Pre-coated

Protocol: Semaphorin 3A (SEMA3A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3A (SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 3A (SEMA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SEMA3A

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: Semaphorin 3A (SEMA3A) (SEMA3A Products)

Synonyms: COLL1 ELISA Kit, HH16 ELISA Kit, Hsema-I ELISA Kit, Hsema-III ELISA Kit, SEMA1 ELISA Kit, SEMAD ELISA Kit, SEMAIII ELISA Kit, SEMAL ELISA Kit, SemD ELISA Kit, coll-1 ELISA Kit, Semad ELISA Kit, COLL-1 ELISA Kit, SEMA3A ELISA Kit, Dsema3a ELISA Kit, Sema3A ELISA Kit, sema1a ELISA Kit, sema3a1 ELISA Kit, semaZ1a ELISA Kit, sema1b ELISA Kit, sema3a2 ELISA Kit, semaZ1b ELISA Kit, semaphorin 3A ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A ELISA Kit, Sema-3a ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab ELISA Kit, SEMA3A ELISA Kit, Sema3a ELISA Kit, Sema-3a ELISA Kit, sema3aa ELISA Kit, sema3ab ELISA Kit

Pathways: Regulation of Cell Size