SEMA4B ELISA Kit

Catalog No. ABIN2044519

Product Details SEMA4B ELISA Kit

Target: SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.071 ng/mL

Application Details

Plate: Pre-coated

Protocol: Semaphorin 4B (SEMA4B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4B (SEMA4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4B (SEMA4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4B (SEMA4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 4B (SEMA4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SEMA4B

Target: SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))

Alternative Name: Semaphorin 4B (SEMA4B) (SEMA4B Products)

Synonyms: sema4b ELISA Kit, MGC80974 ELISA Kit, SEMA4B ELISA Kit, SEMAC ELISA Kit, SemC ELISA Kit, Semac ELISA Kit, mKIAA1745 ELISA Kit, semaphorin 4B ELISA Kit, semaphorin 4B S homeolog ELISA Kit, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B ELISA Kit, SEMA4B ELISA Kit, sema4b.S ELISA Kit, Sema4b ELISA Kit