SPINK5 ELISA Kit

Catalog No. ABIN2044531

Product Details SPINK5 ELISA Kit

Target: SPINK5 (serine Peptidase Inhibitor, Kazal Type 5 (SPINK5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 28 pg/mL

Application Details

Plate: Pre-coated

Protocol: Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SPINK5

Target: SPINK5 (serine Peptidase Inhibitor, Kazal Type 5 (SPINK5))

Alternative Name: Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) (SPINK5 Products)

Synonyms: LEKTI ELISA Kit, LETKI ELISA Kit, NETS ELISA Kit, NS ELISA Kit, VAKTI ELISA Kit, 2310065D10Rik ELISA Kit, AV238912 ELISA Kit, LEKT1 ELISA Kit, LETK1 ELISA Kit, VAKT1 ELISA Kit, serine peptidase inhibitor, Kazal type 5 ELISA Kit, Spink5 ELISA Kit, SPINK5 ELISA Kit