SIRPG ELISA Kit

Catalog No. ABIN2044557

Product Details SIRPG ELISA Kit

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Signal Regulatory Protein Gamma (SIRPg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Gamma (SIRPg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Gamma (SIRPg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Gamma (SIRPg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Signal Regulatory Protein Gamma (SIRPg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SIRPG

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Alternative Name: Signal Regulatory Protein gamma (SIRPg) (SIRPG Products)

Synonyms: CD172g ELISA Kit, SIRP-B2 ELISA Kit, SIRPB2 ELISA Kit, SIRPgamma ELISA Kit, bA77C3.1 ELISA Kit, SIRPG ELISA Kit, signal regulatory protein gamma ELISA Kit, signal-regulatory protein gamma ELISA Kit, SIRPG ELISA Kit, LOC716034 ELISA Kit, LOC102138723 ELISA Kit