SLC9A3 ELISA Kit

Catalog No. ABIN2044571

Product Details SLC9A3 ELISA Kit

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: Sodium/Hydrogen Exchanger 3 (NHE3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 3 (NHE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sodium/Hydrogen Exchanger 3 (NHE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SLC9A3

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Alternative Name: Sodium/Hydrogen Exchanger 3 (NHE3) (SLC9A3 Products)

Synonyms: SLC9A3 ELISA Kit, 9030624O13Rik ELISA Kit, AI930210 ELISA Kit, NHE-3 ELISA Kit, NHE3 ELISA Kit, Nhe3 ELISA Kit, solute carrier family 9 member A3 ELISA Kit, sodium/hydrogen exchanger 3 ELISA Kit, Sodium/hydrogen exchanger 3 ELISA Kit, putative sodium/hydrogen exchanger 3 (nhe3) ELISA Kit, solute carrier family 9 (sodium/hydrogen exchanger), member 3 ELISA Kit, SLC9A3 ELISA Kit, LOC5570922 ELISA Kit, GL50803_102647 ELISA Kit, Smp_045680 ELISA Kit, Slc9a3 ELISA Kit

Pathways: Proton Transport