SDHB ELISA Kit

Catalog No. ABIN2044621

Product Details SDHB ELISA Kit

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Succinate Dehydrogenase Complex Subunit B (SDHB) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit B (SDHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit B (SDHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit B (SDHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Succinate Dehydrogenase Complex Subunit B (SDHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Alternative Name: Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB Products)

Synonyms: succinate dehydrogenase 2-3 ELISA Kit, CG3283 ELISA Kit, Dmel\\CG3283 ELISA Kit, Ip ELISA Kit, SDH ELISA Kit, SDH-IP ELISA Kit, SDH-Ip ELISA Kit, SDHb ELISA Kit, sdhB ELISA Kit, CWS2 ELISA Kit, IP ELISA Kit, PGL4 ELISA Kit, SDH1 ELISA Kit, SDH2 ELISA Kit, SDHIP ELISA Kit, 0710008N11Rik ELISA Kit, fj46d06 ELISA Kit, wu:fj46d06 ELISA Kit, succinate dehydrogenase complex iron sulfur subunit B ELISA Kit, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 ELISA Kit, Succinate dehydrogenase, subunit B (iron-sulfur) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) ELISA Kit, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog ELISA Kit, SDHB ELISA Kit, SDH2-3 ELISA Kit, SdhB ELISA Kit, sdhb ELISA Kit, Sdhb ELISA Kit, sdhb.S ELISA Kit