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T-Bet ELISA Kit

Reactivity: Human Colorimetric Sandwich ELISA 0.15-10 ng/mL
Catalog No. ABIN2044645
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    T-Bet
    Reactivity
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.15-10 ng/mL
    Minimum Detection Limit
    0.15 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.052 ng/mL
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  • Plate
    Pre-coated
    Protocol
    T-Box Protein 21 (TBX21) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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    T-Bet
    Alternative Name
    T-Box Protein 21 (TBX21) (T-Bet Products)
    Synonyms
    T-PET ELISA Kit, T-bet ELISA Kit, TBET ELISA Kit, TBLYM ELISA Kit, TBT1 ELISA Kit, Tbet ELISA Kit, Tblym ELISA Kit, T-box 21 ELISA Kit, TBX21 ELISA Kit, Tbx21 ELISA Kit
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