WNT5A ELISA Kit

Catalog No. ABIN2044831

Product Details WNT5A ELISA Kit

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) (WNT5A Products)

Synonyms: hWNT5A ELISA Kit, wnt-5A ELISA Kit, wnt5a-B ELISA Kit, WNT5A ELISA Kit, 8030457G12Rik ELISA Kit, Wnt-5a ELISA Kit, Wnt family member 5A ELISA Kit, Wnt family member 5A S homeolog ELISA Kit, wingless-type MMTV integration site family, member 5a ELISA Kit, wingless-type MMTV integration site family, member 5A ELISA Kit, wingless-related MMTV integration site 5A ELISA Kit, Wnt signaling ligand ELISA Kit, WNT5A ELISA Kit, Wnt5a ELISA Kit, wnt5a.S ELISA Kit, wnt5a ELISA Kit, LOC578814 ELISA Kit, wnt5 ELISA Kit

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation