ACER3 ELISA Kit (Alkaline Ceramidase 3)

Details for Product ACER3 ELISA Kit No. ABIN2065209, Supplier: Log in to see
Antigen
  • phca
  • PHCA
  • APHC
  • 1110057L18Rik
  • 5430429L08Rik
  • AV015045
  • Phca
  • RGD1561254
  • alkaline ceramidase 3 S homeolog
  • alkaline ceramidase 3
  • acer3.S
  • ACER3
  • acer3
  • Acer3
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
1
1
Method Type
Cell ELISA
Application
ELISA
Options
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Sample Type Cell Culture Cells
Analytical Method Quantitative
Detection Method Colorimetric
Characteristics Assay Type: Cell-Based
Alternative Name PHCA (ACER3 ELISA Kit Abstract)
Background Synonyms: Alkaline phytoceramidase, aPHC, Alkaline ceramidase, Alkaline dihydroceramidase SB89
Gene Symbol: ACER3
Gene ID 55331
UniProt Q9NUN7
Plate Uncoated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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