IPMK ELISA Kit (Inositol Polyphosphate Multikinase)

Details for Product IPMK ELISA Kit No. ABIN2114200, Supplier: Log in to see
  • MGC83832
  • IPMK
  • zgc:158331
  • ipmk
  • si:dkey-223l5.1
  • 2410017C19Rik
  • AA408208
  • Impk
  • Ipk2
  • inositol polyphosphate multikinase
  • inositol polyphosphate multikinase b
  • inositol polyphosphate multikinase a
  • ipmk
  • IPMK
  • ipmkb
  • EDI_171250
  • CC1G_06147
  • CpipJ_CPIJ006477
  • CpipJ_CPIJ016779
  • PAAG_02384
  • MCYG_03683
  • ipmka
  • EDI_070480
  • VDBG_01275
  • MGYG_03269
  • Tsp_05937
  • Ipmk
Human, Mouse (Murine), Rat (Rattus)
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Analytical Method Quantitative
Detection Method Colorimetric
Characteristics Assay Type: Cell-Based
Alternative Name IPMK (IPMK ELISA Kit Abstract)
Background Synonyms: Inositol polyphosphate multikinase, Inositol 1, 3, 4, 6-tetrakisphosphate 5-kinase , IPMK , IMPK
Gene Symbol: IPMK
Gene ID 253430
UniProt Q8NFU5
Research Area Stem Cells, Cell Cycle, Kinases/Phosphatases
Pathways Tube Formation
Plate Uncoated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months
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